| Antibody Type: | Polyclonal |
| Isotype: | Rabbit IgG |
| Purification: | Affinity Purified |
|
| Appl.: | WB |
| Reactivity: | Human |
| Molecular Weight: | 95 kDa |
|
| Western Blot |
Chromatin IP |
| 1:500 - 1:1,000 |
Not tested |
Background for NBS1 antibody (pAb):
NBS1 (
Nijmegen breakage syndrome 1,
NBN or
p95) is a component of the
MRE11/RAD50/NBN (
MRN complex), which plays a critical role in the cellular response to
DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3′-5′ exonuclease activity, which are provided by MRE11A. NBN modulate the DNA damage signal sensing by recruiting
PI3-kinase/PI4-kinase family members
ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions.
NBS1 (
NBN) also functions in telomere length maintenance by generating the 3′ overhang which serves as a primer for telomerase dependent telomere elongation.
NBS1 (
NBN) is a major player in the control of intra-S-phase checkpoint and there is some evidence that
NBS1 (
NBN) is involved in G
1 and G
2 checkpoints. The roles of
NBS1 (
NBN)/
MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability.
Immunogen for NBS1 antibody (pAb):
Peptide corresponding to the C-terminus of human NBS1.
Application Notes for NBS1 antibody (pAb):
This NBS1 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).
Buffer:
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.
Positive Control:
Active Motif's
HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for NBS1 antibody.
Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
NBS1 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with NBS1 pAb (1:500 dilution).
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee:
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
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