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Histone H3 monomethyl Lys27 antibody (pAb)
| Catalog No: 39377 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39378 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:500 - 1:2,000 | — | 1:500 - 1:2,000 |
for Histone H3 monomethyl Lys27 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.
for Histone H3 monomethyl Lys27 antibody (pAb)Immunogen
This Histone H3 monomethyl Lys27 antibody was raised against a peptide including monomethyl-lysine 27 of human histone H3.
for Histone H3 monomethyl Lys27 antibody (pAb)Application Notes
This Histone H3 monomethyl Lys27 antibody (H3K27me) has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and immunofluorescence (1:500 - 1:2,000 dilution).
for Histone H3 monomethyl Lys27 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 monomethyl Lys27 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 monomethyl Lys27 antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 monomethyl Lys27 antibody (pAb).
Histone H3 monomethyl Lys27 antibody tested by Western blot.
HeLa acid extract (10 µg per lane) probed with Histone H3 monomethyl Lys27 antibody (1:2,000 dilution).
Histone H3 monomethyl Lys27 antibody tested by immunofluorescence.
Top left: HeLa cells stained with Histone H3 monomethyl Lys27 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.
Histone H3 monomethyl Lys27 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys27 pAb for monomethyl Lys27 histone H3. Methylated peptides corresponding to the immunogen and related sequences derived from histone H3 were spotted onto PVDF and probed with the antibody at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: unmodified Lys4 peptide.
Lane 2: monomethyl Lys4.
Lane 3: dimethyl Lys4; Lane 4: trimethyl Lys4.
Lane 5: monomethyl Lys9.
Lane 6: unmodified Lys9.
Lane 7: dimethyl Lys9.
Lane 8: trimethyl Lys9.
Lane 9: unmodified Lys27.
Lane 10: monomethyl Lys27.
Lane 11: dimethyl Lys27.
Lane 12: trimethyl Lys27.
for Histone H3 monomethyl Lys27 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 monomethyl Lys27 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
