MAD2L1 antibody (mAb)

for MAD2L1 antibody (mAb) (Clone 17D10)Background

MAD2L1 (aka HSMAD2, MAD2, MAD2-like 1) is the human homolog of the budding yeast MAD2, or mitotic arrest deficient 2 gene. MAD2L1 is a component of the mitotic spindle assembly checkpoint, which monitors the attachment of the mitotic spindle to the kinetochores of segregating chromosomes and delays the onset of anaphase until attachment is complete and the chromosomes are properly aligned on the metaphase plate. MAD2L1 associates only with unattached kinetochores in prometaphase, and prevents cell cycle progression by associating with the Anaphase Promoting Complex (APC), specifically restricting the activity of the CDC20 protein.

for MAD2L1 antibody (mAb) (Clone 17D10)Immunogen

Full-length recombinant human MAD2L1.

for MAD2L1 antibody (mAb) (Clone 17D10)Application Notes

This MAD2L1 antibody (Clone 17D10) has been validated for use in Western blotting (2 - 5 µg/ml dilution).

for MAD2L1 antibody (mAb) (Clone 17D10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.

for MAD2L1 antibody (mAb) (Clone 17D10)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for MAD2L1 antibody (Clone 17D10).

for MAD2L1 antibody (mAb) (Clone 17D10)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MAD2L1 antibody (mAb) (Clone 17D10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.