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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Background
c-Myc (or cMyc) is a transcription factor that regulates gene expression. Myc family members contain a basic Helix-Loop-Helix leucine zipper domain. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.
for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Immunogen
This c-Myc antibody was raised against a synthetic peptide corresponding to amino acid residues 408-420 of human c-Myc; it recognizes a 61 kDa form of c-Myc.
for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Application Notes
This c-Myc antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at 37°C. Individual optimization may be required.
for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Buffer
PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.
for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Positive Control
Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for c-Myc antibody (61 kDa form).
c-Myc mAb (61 kDa form) tested by Western blot.
Detection of c-Myc by Western blot analysis. A 61 kDa form of c-Myc is detected in nuclear extracts derived from K-562 cells using c-Myc mouse monoclonal antibody. Lanes 1-3 represent serial dilutions of the monoclonal antibody (1/500, 1/1000 and 1/2500).
for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting


