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Antibodies

c-Myc antibody (mAb) (61 kDa form)

Clone: 9E11
Catalog No: 39502 Format: 100 µl $340 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG1
Purification:Culture Supernatant
Applications:WB
Reactivity:Human
Molecular Weight:61 kDa

Applications Table

Western Blot Chromatin IP
1:500 - 1:1,000

for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Background

c-Myc (or cMyc) is a transcription factor that regulates gene expression. Myc family members contain a basic Helix-Loop-Helix leucine zipper domain. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.

for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Immunogen

This c-Myc antibody was raised against a synthetic peptide corresponding to amino acid residues 408-420 of human c-Myc; it recognizes a 61 kDa form of c-Myc.

for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Application Notes

This c-Myc antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution). For optimal results, primary antibody incubations should be performed at 37°C. Individual optimization may be required.

for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Buffer

PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic.

for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for c-Myc antibody (61 kDa form).

 
c-Myc antibody (mAb) (61 kDa form) tested by Western blot.

c-Myc mAb (61 kDa form) tested by Western blot.
Detection of c-Myc by Western blot analysis. A 61 kDa form of c-Myc is detected in nuclear extracts derived from K-562 cells using c-Myc mouse monoclonal antibody. Lanes 1-3 represent serial dilutions of the monoclonal antibody (1/500, 1/1000 and 1/2500).

for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Storage

Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for c-Myc antibody (mAb) (61 kDa form) (Clone 9E11)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

c-Myc antibody (mAb) (61 kDa form)
 

Data Thumbnails

c-Myc antibody (mAb) (61 kDa form) tested by Western blot.

Western blot of c-Myc mAb (61 kDa form).
(Click image to enlarge and see details).