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Antibodies

HDAC1 antibody (mAb)

Clone: 10E2
Aliases: HD1, RPD3, GON-10, RPD3L1
Catalog No: 39531 Format: 100 µl $410 Buy Now
Catalog No: 39532 Format: 10 µl $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Culture Supernatant
Host:Mouse
Molecular Weight:60 kDa
Reactivity:Human, Mouse, Rat

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

for HDAC1 antibody (mAb) (Clone 10E2)Application Notes


Validated Applications:
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP
IP
IHC
IF

for HDAC1 antibody (mAb) (Clone 10E2)Immunogen

This HDAC1 antibody was raised against a KLH-conjugated peptide corresponding to amino acids 467-482 of human and murine HDAC1.

for HDAC1 antibody (mAb) (Clone 10E2)Buffer

Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39881) of this antibody that was purified by Protein A Chromatography is also available.

for HDAC1 antibody (mAb) (Clone 10E2)References

 
HDAC1 antibody (mAb) tested by immunofluorescence.

HDAC1 antibody (mAb) (Clone 10E2) tested by immunofluorescence.
Top left: HeLa cells stained with HDAC1 antibody (mAb) (Clone 10E2) (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

HDAC1 antibody (mAb) tested by Western blot.

HDAC1 antibody (mAb) (Clone 10E2) tested by Western blot.
Detection of HDAC1 by Western blot analysis.Nuclear extract of HeLa cells (30μg) was probed with HDAC1 antibody (mAb) (Clone 10E2) at a 1:500 dilution.

for HDAC1 antibody (mAb) (Clone 10E2)Background

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for HDAC1 antibody (mAb) (Clone 10E2)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HDAC1 antibody (Clone 10E2).

for HDAC1 antibody (mAb) (Clone 10E2)qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with HDAC1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use HDAC1 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HDAC1 antibody (mAb).

for HDAC1 antibody (mAb) (Clone 10E2)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HDAC1 antibody (mAb) (Clone 10E2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HDAC1 antibody (mAb)
 

Data Thumbnails

HDAC1 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of HDAC1 mAb.
(Click image to enlarge and see details.)

HDAC1 antibody (mAb) tested by Western blot.

Western blot of HDAC1 mAb.
(Click image to enlarge and see details.)