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Antibodies

HDAC2 antibody (mAb)

Clone: 3F3
Aliases: HD2, RPD3, YAF1
Catalog No: 39533 Format: 200 µl $410 Buy Now
Catalog No: 39534 Format: 10 µl $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Culture Supernatant
Host:Mouse
Molecular Weight:55 kDa
Reactivity:Human, Mouse, Rat

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for HDAC2 antibody (mAb) (Clone 3F3)Application Notes


Validated Applications:
WB: 1:250 - 1:1,000 dilution
ICC/IF: 1:1,000 - 1:5,000 dilution

Published Applications:
ChIP
IF

for HDAC2 antibody (mAb) (Clone 3F3)Immunogen

This HDAC2 antibody was raised against a KLH-conjugated peptide corresponding to amino acids 473-488 of human HDAC2.

for HDAC2 antibody (mAb) (Clone 3F3)Buffer

Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39883) of this antibody that was purified by Protein G Chromatography is also available.

for HDAC2 antibody (mAb) (Clone 3F3)References

 
HDAC2 antibody (mAb) tested by immunofluorescence.

HDAC2 mAb (Clone 3F3) tested by immunofluorescence.
Detection of HDAC2 by immunofluorescence. HeLa cells were stained with HDAC2 mAb (Clone 3F3) at a 1:1,000 dilution.
Red: HDAC2 mAb (Clone 3F3)
Green: alpha-Tubulin mouse monoclonal (Clone 5-B-1-2) conjugated to Chromeo™ 488.

HDAC2 antibody (mAb) tested by Western blot.

HDAC2 mAb (Clone 3F3) tested by Western blot.
Detection of HDAC2 by Western blot. The analysis was performed using HeLa nuclear extract and HDAC2 mAb (Clone 3F3) at a 1:5,000 dilution.

for HDAC2 antibody (mAb) (Clone 3F3)Background

HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for HDAC2 antibody (mAb) (Clone 3F3)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HDAC2 antibody (Clone 3F3).

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with HDAC2 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use HDAC2 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HDAC2 antibody (mAb).

for HDAC2 antibody (mAb) (Clone 3F3)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HDAC2 antibody (mAb) (Clone 3F3)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HDAC2 antibody (mAb)
 

Data Thumbnails

HDAC2 antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of HDAC2 mAb.
(Click image to enlarge and see details.)

HDAC2 antibody (mAb) tested by Western blot.

Western blot of HDAC2 mAb.
(Click image to enlarge and see details.)