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Antibodies

HIRA antibody (mAb)

Clone: WC15
Aliases: TUP1, DGCR1, TUPLE1
Catalog No: 39555 Format: 200 µg $410 Buy Now
Catalog No: 39556 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:120 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated

for HIRA antibody (mAb) (Clone WC15)Application Notes


Validated Applications:
IP: 5 µg per IP
WB: 2 - 5 µg/ml dilution

for HIRA antibody (mAb) (Clone WC15)Immunogen

This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.

for HIRA antibody (mAb) (Clone WC15)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HIRA antibody (mAb) (Clone WC15)References

 
HIRA antibody (mAb) tested by Western blot.

HIRA mAb (Clone WC15) tested by Western blot.
5 µg of HIRA mAb (Clone WC15) was used to immunoprecipitate HIRA from 500 µg of colcemid-treated U2OS nuclear extract (lane 2). 5 µg of control mouse IgG was also used (lane 1). The immunoprecipitated protein was detected by Western blotting using HIRA mAb (Clone WC119.2H11) at a dilution of 5 µg/ ml.

for HIRA antibody (mAb) (Clone WC15)Background

HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1α is involved in the formation of the senescence-associated heterochromatin foci.

for HIRA antibody (mAb) (Clone WC15)Positive Control

Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for HIRA (Clone WC15) antibody.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use HIRA antibody (mAb).

for HIRA antibody (mAb) (Clone WC15)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HIRA antibody (mAb) (Clone WC15)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HIRA antibody (mAb)
 

Data Thumbnails

HIRA antibody (mAb) tested by Western blot.

Western blot of HIRA mAb.
(Click image to enlarge and see details.)