HIRA antibody (mAb)

RRID: AB_2793256
Clone: WC119.2H11
Aliases: TUP1, DGCR1, TUPLE1
Catalog No: 39557 Format: 100 µg $500 Buy
Catalog No: 39558 Format: 10 µg $125 Buy
Catalog No: 39457 Format: 50 µg $285 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
120 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
WB: 1 - 5 µg/ml dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ICC/IF
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

HIRA mAb (Clone WC119.2H11) tested by Western blot.
5 µg of HIRA mAb (Clone WC15) was used to immunoprecipitate HIRA from 500 µg of colcemid-treated U2OS nuclear extract (lane 2). 5 µg of control mouse IgG was also used (lane 1). The immunoprecipitated protein was detected by Western blotting using HIRA mAb (Clone WC119.2H11) at a dilution of 5 µg/ ml.

Background

HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1α is involved in the formation of the senescence-associated heterochromatin foci.

Positive Control

Active Motif's HeLa acid extract (PTX treated) (Catalog No. 36201) can be used as a positive control for HIRA antibody (Clone WC119.2H11).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting