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PARP-1 C-terminal antibody (pAb)
| Catalog No: 39561 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39562 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 1:1,000 - 1:6,000 | 10 µl per ChIP | Published. See Application Notes below. |
for PARP-1 C-terminal antibody (pAb)Background
PARP-1 C-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis.
For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website.
for PARP-1 C-terminal antibody (pAb)Immunogen
His-tagged fusion protein corresponding to the C-terminal half of human PARP-1 was used to generate this PARP-1 antibody.
for PARP-1 C-terminal antibody (pAb)Application Notes
This PARP-1 C-terminal antibody has been validated for use in Western blotting (1:1,000 - 1:6,000 dilution), chromatin IP (ChIP, 10 µl per ChIP) and reported to work in immunohistochemistry and immunoprecipitation. See references for additional information.
for PARP-1 C-terminal antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for PARP-1 C-terminal antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PARP-1 C-terminal antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with PARP-1 C-terminal antibody (pAb).
for PARP-1 C-terminal antibody (pAb)References
This antibody has been used in the following references:
Kim MY et al (2004) Cell 119: 803-814.
Krishnakumar R et al (2008) Science 319: 819-821.
PARP-1 C-terminal antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with PARP-1 C-terminal antibody at a dilution of 1:6,000.
PARP-1 C-terminal antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and MCF7 chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µl of PARP-1 C-terminal antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the BRCA2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for PARP-1 C-terminal antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for PARP-1 C-terminal antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
