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Antibodies

PARP-1 C-terminal antibody (pAb)

Aliases: PARP, PPOL, ADPRT, ARTD1, ADPRT1, ADPRT 1, pADPRT-1
Catalog No: 39561 Format: 200 µl $410 Buy Now
Catalog No: 39562 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:120 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated

for PARP-1 C-terminal antibody (pAb)Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
WB: 1:1,000 - 1:6,000 dilution

Published Applications: see references

for PARP-1 C-terminal antibody (pAb)Immunogen

This PARP-1 C-terminal antibody was raised against a His-Tagged fusion protein corresponding to the C-terminal half of human PARP-1 was used to generate this PARP-1 antibody.

for PARP-1 C-terminal antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PARP-1 C-terminal antibody (pAb)References

 
PARP-1 C-terminal antibody (pAb) tested by ChIP.

PARP-1 C-terminal antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of PARP-1 C-terminal antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the BRCA2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

PARP-1 C-terminal antibody (pAb) tested by Western blot.

PARP-1 C-terminal antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with PARP-1 C-terminal antibody at a dilution of 1:6,000.

for PARP-1 C-terminal antibody (pAb)Background

PARP-1 C-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis.

For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section or our website.

for PARP-1 C-terminal antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PARP-1 C-terminal antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PARP-1 C-terminal antibody (pAb).

for PARP-1 C-terminal antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PARP-1 C-terminal antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PARP-1 C-terminal antibody (pAb)
 

Data Thumbnails

PARP-1 C-terminal antibody (pAb) tested by ChIP.

ChIP of PARP-1 C-terminal pAb.
(Click image to enlarge and see details.)

PARP-1 C-terminal antibody (pAb) tested by Western blot.

Western blot of PARP-1 C-terminal pAb.
(Click image to enlarge and see details.)