Histone H3.cs1 antibody (pAb)

for Histone H3.cs1 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Histone H3 is proteolytically cleaved at its N-terminus by Cathepsin L during the differentiation of embryonic stem cells. It is possible that this processing occurs in order to remove modifications that serve as protein recognition elements (e.g. Histone H3 trimethyl Lys9) present in the extreme N-terminus.

for Histone H3.cs1 antibody (pAb)Immunogen

Peptide including the site of Cathepsin L cleavage at the N-terminus.

for Histone H3.cs1 antibody (pAb)Application Notes

This Histone H3.cs1 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).

Depending upon the gel system employed, a change in mobility of the clipped form of histone H3 may not be observed.

for Histone H3.cs1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol, 0.035% sodium azide.

for Histone H3.cs1 antibody (pAb)References

Duncan et al (2008) Cell 135:284-294.

for Histone H3.cs1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Histone H3.cs1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.