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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| 1:2,000 - 1:10,000 | — | 1:1,000 - 1:2,500 | 5 µl per IP |
for Histone H1 antibody (pAb)Background
The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation and play a major role in regulating gene expression.
for Histone H1 antibody (pAb)Immunogen
This antibody was raised against a recombinant protein corresponding to full-length Drosophila Histone H1.
for Histone H1 antibody (pAb)Application Notes
This Histone H1 antibody has been validated for use in Western blotting (1:2,000 - 1:10,000 dilution), immunofluorescence (1:1,000 - 1:2,500 dilution) and immunoprecipitation (5 µl per IP). Addition of 0.05% Tween 20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
for Histone H1 antibody (pAb)Buffer
PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol.
for Histone H1 antibody (pAb)Positive Control
Active Motif's Schneider's Drosophila L2 nuclear extract (Catalog No. 36087) can be used as a positive control for Histone H1 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H1 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H1 antibody (pAb).
for Histone H1 antibody (pAb)References
This antibody has been used in the following references:
ChIP: Lu X et al (2009) Genes Dev 23: 452-465.
Histone H1 pAb tested by Western blot.
Detection of Histone H1 by Western blot. The analysis was performed using Schneider's Drosophila L2 nuclear extract and Histone H1 pAb at a 1:5,000 dilution.
for Histone H1 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
