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Histone H4 acetyl Lys5 antibody (pAb)
| Catalog No: 39583 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39584 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:2,500 - 1:10,000 | 5 - 10 µl per ChIP | 1:500 - 1:1,000 |
for Histone H4 acetyl Lys5 antibody (pAb)Background
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. H4 molecules acetylated at Lys5 or Lys8 are distributed in overlapping, but non-identical, islands throughout the euchromatic chromosome arms.
for Histone H4 acetyl Lys5 antibody (pAb)Immunogen
This Histone H4 acetyl Lys5 antibody was raised against a peptide including acetyl-lysine 5 of histone H4.
for Histone H4 acetyl Lys5 antibody (pAb)Application Notes
This Histone H4 acetyl Lys5 antibody (H4K5Ac) has been validated for use in Western blotting (1:2,500 - 1:10,000 dilution), chromatin IP (5 - 10 µl per ChIP) and immunofluorescence (1:500 - 1:1,000 dilution).
for Histone H4 acetyl Lys5 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol, 0.035% sodium azide.
for Histone H4 acetyl Lys5 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 acetyl Lys5 antibody.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4 acetyl Lys5 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H4 acetyl Lys5 antibody (pAb).
Histone H4 acetyl Lys5 antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H4 acetyl Lys5 polyclonal antibody (1:5,000).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate.
Histone H4 acetyl Lys5 antibody tested by immunofluorescence.
Top left: HeLa cells stained with Histone H4 acetyl Lys5 antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Stained with DAPI. Bottom right: Merge of all 3 images.
Histone H4 acetyl Lys5 antibody tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 antibody for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys5 peptide.
Lane 2: unmodified Lys5 peptide.
Lane 3: acetyl-Lys8 peptide.
Lane 4: unmodified Lys8 peptide.
Lane 5: acetyl-Lys12 peptide.
Lane 6: unmodified Lys12 peptide.
Lane 7: acetyl-Lys16 peptide.
Lane 8: unmodified Lys16 peptide.
Histone H4 acetyl Lys5 antibody tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and 50 µl of Ready-to-ChIP HeLa Chromatin (Catalog No. 53015) per ChIP. Subsequent to the ChIP reaction, DNA was purified from the immunoprecipitated chromatin and a region of the human GAPDH promoter was amplified by PCR.
Lane 1: PCR input control.
Lane 2: ChIP using negative control rabbit IgG.
Lane 3: ChIP using 10 µl of Histone H4 acetyl Lys5 antibody.
Histone H4 acetyl Lys5 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 µg of Histone H4 acetyl Lys5 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
for Histone H4 acetyl Lys5 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 acetyl Lys5 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
