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PXR antibody (pAb)
| Catalog No: 39589 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39590 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for PXR antibody (pAb)Background
PXR (NR1I2, Pregnane X receptor, nuclear receptor subfamily 1 group I member 2) belongs to a family of transcription factors characterized by a ligand-binding domain and a DNA-binding domain. PXR is an orphan receptor that regulates transcription of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR (RXR-α and RXR-β). PXR is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin.
for PXR antibody (pAb)Immunogen
This PXR antibody was raised against a peptide containing amino acid residues 57-71 of human PXR.
for PXR antibody (pAb)Application Notes
This PXR antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution).
for PXR antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.
for PXR antibody (pAb)Positive Control
Active Motif's Hep G2 nuclear extract (Catalog No. 36011) can be used as a positive control for PXR antibody.
PXR pAb tested by Western blot.
Hep G2 nuclear extract (20 µg per lane) was probed with PXR pAb at a dilution of 1:1,000.
for PXR antibody (pAb)Storage
Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for PXR antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
