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Histone H2A, C-terminal antibody (pAb)
| Catalog No: 39591 | Format: 200 µl | $340 | Buy Now |
| Catalog No: 39592 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:1,000 | — |
for Histone H2A, C-terminal antibody (pAb)Background
Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (Histone H2A, Histone H2B, Histone H3, and Histone H4).
for Histone H2A, C-terminal antibody (pAb)Immunogen
Peptide containing the C- terminal region of human histone H2A.
for Histone H2A, C-terminal antibody (pAb)Application Notes
This Histone H2A, C-terminal antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).
for Histone H2A, C-terminal antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.
for Histone H2A, C-terminal antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H2A, C-terminal antibody.
Histone H2A, C-terminal pAb tested by Western blot.
Detection of Histone H2A by Western blot. The analysis was performed using HeLa acid extract (10 µg, lane 1) and recombinant histone H2A protein (200 ng, lane 2) probed with Histone H2A, C-terminal pAb at a 1:500 dilution).
for Histone H2A, C-terminal antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H2A, C-terminal antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
