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Antibodies

HMGA1 antibody (pAb)

Aliases: HMG-R, HMGIY, HMGA1A
Catalog No: 39615 Format: 100 µl $410 Buy Now
Catalog No: 39650 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:20 kDa
Reactivity:Human

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for HMGA1 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:1,000 dilution
WB: 1:500 - 1:2,000 dilution

for HMGA1 antibody (pAb)Immunogen

This HMGA1 antibody was raised against a peptide corresponding to the N-terminus of human HMGA1.

for HMGA1 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HMGA1 antibody (pAb)References

 
HMGA1 antibody (pAb) tested by immunofluorescence.

HMGA1 antibody tested by immunofluorescence.
Detection of HMGA1 by immunofluorescence. HeLa cells were stained with HMGA1 antibody at a dilution of 1:1,000. Top panel: HMGA1 antibody staining. Middle panel: DAPI. Bottom panel: merge.

HMGA1 antibody (pAb) tested by Western blot.

HMGA1 antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with HMGA1 pAb (1:500 dilution). Lane 1: no treatment.

for HMGA1 antibody (pAb)Background

High mobility group (HMG) proteins are nuclear proteins that may significantly affect DNA interactions by altering double-stranded DNA structure. HMGA1 is frequently acetylated and is found in the nucleus.

for HMGA1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HMGA1 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HMGA1 antibody (pAb).

for HMGA1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HMGA1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HMGA1 antibody (pAb)
 

Data Thumbnails

HMGA1 antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of HMGA1 pAb.
(Click image to enlarge and see details.)

HMGA1 antibody (pAb) tested by Western blot.

Western blot of HMGA1 pAb.
(Click image to enlarge and see details.)