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HMGA1 antibody (pAb)
| Catalog No: 39615 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39650 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:500 - 1:2,000 | — | 1:1,000 |
for HMGA1 antibody (pAb)Background
High mobility group (HMG) proteins are nuclear proteins that may significantly affect DNA interactions by altering double-stranded DNA structure. HMGA1 is frequently acetylated and is found in the nucleus.
for HMGA1 antibody (pAb)Immunogen
This HMGA1 antibody was raised against a peptide corresponding to the N-terminus of human HMGA1.
for HMGA1 antibody (pAb)Application Notes
This HMGA1 antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and immunofluorescence (1:1,000).
for HMGA1 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for HMGA1 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HMGA1 antibody.
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HMGA1 antibody (pAb).
HMGA1 antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with HMGA1 pAb (1:500 dilution). Lane 1: no treatment.
HMGA1 antibody tested by immunofluorescence.
Detection of HMGA1 by immunofluorescence. HeLa cells were stained with HMGA1 antibody at a dilution of 1:1,000. Top panel: HMGA1 antibody staining. Middle panel: DAPI. Bottom panel: merge.
for HMGA1 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for HMGA1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
