YOUR CART
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| Western Blot | Chromatin IP |
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| 0.1 - 1 µg/ml | 0.2 - 2 µg per ChIP |
| View Technical Data Sheet (TDS) |
Background for CTCF antibody (mAb) CCCTC-binding factor:
The vertebrate insulator protein, CCCTC binding factor (CTCF), is a highly conserved, ubiquitously expressed zinc-finger DNA-binding protein that has widespread functions in gene regulation, chromatin organization and epigenetic imprinting. CTCF plays dual roles in cell growth regulatory processes and epigenetic functions. CTCF is involved in different aspects of gene regulation including promoter activation and repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF can modulate higher-order chromatin structure by regulating long-distance interactions between proteins bound at discrete regulatory elements.Chromatin IP Assays:
Active Motif has Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page
Immunogen for CTCF antibody (mAb) CCCTC-binding factor:
This CTCF antibody (mAb) was raised recombinant human CTCF expressed in Pichia pastoris.Application Notes for CTCF antibody (mAb) CCCTC-binding factor:
This CTCF antibody (mAb) has been validated for use in Western blotting (0.1 - 1 µg/ml) and chromatin IP (0.2 - 2 µg per ChIP).The development of this antibody is described in Reference 1 (Kim, et al, 2007).
Buffer:
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.Positive Control:
Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for CTCF antibody.Compatible Secondary Antibodies:
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for your experiments.
References:
1. Kim T.H.et al (2007) Cell 128: 1231–1245.
CTCF antibody (mAb) tested by Western blot.
Nuclear extract of HEK293 cells (20 µg per lane) probed with CTCF antibody (mAb) at a dilution of 0.25 µg/ml.
CTCF antibody (mAb) tested by ChIP.
Chromatin IP performed using the ChIP-IT™ Express Kit (Catalog No. 53008) and 25 µl of Ready-to-ChIP HeLa Chromatin (Catalog No. 53015) per ChIP using 2 µg of CTCF mAb or 2 µg of mouse IgG (IgG) as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the Imprinting Control Region (ICR) of the IGF2/H19 locus. The data presented are expressed as a percentage of the starting material (ChIP/input).
Storage:
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.Guarantee:
This product is guaranteed for 6 months from date of receipt.This product is for research use only and is not for use in diagnostic procedures.
Application Key:
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- IF = Immunofluorescence;
- ICC = Immunocytochemistry;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
