STAT1 phospho Ser727 antibody (pAb)

for STAT1 phospho Ser727 antibody (pAb)Background

STAT1 is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT1 is alternatively spliced to give rise to STAT1α and STAT1β, both of which mediate interferon α activity, but only STAT1α mediates interferon-γ activity. STAT1 plays a critical role in various forms of cell death, both apoptotic and non-apoptotic. The mechanisms of action are both by transcriptional activation of target genes, and by direct association with apoptotic regulators, including TRADD, p53, and HDAC (HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6 and HDAC11). STAT1 target genes include the pro-inflammatory genes for iNOS, COX, VCAM, and ICAM, making STAT1 an attractive anti-inflammatory target.

for STAT1 phospho Ser727 antibody (pAb)Immunogen

This STAT1 phospho Ser727 antibody was raised against a synthetic peptide containing phospho-serine 727 of human STAT1.

for STAT1 phospho Ser727 antibody (pAb)Application Notes

This STAT1 phospho Ser727 antibody has been validated for use in Western blotting (1:500 - 1:1,000 dilution).

for STAT1 phospho Ser727 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol, 0.035% sodium azide.

for STAT1 phospho Ser727 antibody (pAb)Positive Control

Whole-cell extracts from HeLa cells treated with IFNα (2500 U/ml for 30 minutes) can be used as a positive control for STAT1 phospho Ser727 antibody.

for STAT1 phospho Ser727 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for STAT1 phospho Ser727 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.