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Antibodies

Histone H3K4me1 antibody (mAb)

Clone: MABI 0302
Catalog No: 39635 Format: 100 µg $420 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2b
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Dot Blot Validated

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)Application Notes


Validated Applications:
ChIP-Seq: 4 µg each
ChIP: 10 µl per ChIP
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP-Seq

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)Immunogen

The Histone H3 monomethyl Lys4 antibody was raised against a peptide containing monomethyl lysine 4 of human histone H3.

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a PBS only version of this antibody is also available.

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)References

 
Histone H3K4me1 antibody (mAb) tested by ChIP.

Histone H3K4me1 antibody (mAb) tested by ChIP-Seq
ChIP was performed using 10 µg of chromatin from MCF7 cells and 4 µg of antibody. ChIP DNA was sequenced and 19 million sequence tags were mapped to identify Histone H3K4me1 binding sites.

Histone H3K4me1 antibody (mAb) tested by Western blot.

Histone H3 monomethyl Lys4 antibody (mAb) tested by Western blot.
HeLa acid extract probed with Histone H3 monomethyl Lys4 antibody (mAb) (2 µg/ml dilution).

Histone H3K4me1 antibody (mAb) tested by dot blot analysis.

Histone H3 monomethyl Lys4 antibody (mAb) tested by Dot Blot
Dot Blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys4 antibody (mAb) for monomethyl Lys4 histone H3. Methylated peptides corresponding to the immunogen or related peptides were spotted onto PVDF and probed with antibody at 1 µg/ml. Lane 1: unmodified peptide, Lane 2: Monomethyl Lys4, Lanes 3-8: various negative control peptides.

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.

Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 monomethyl Lys4 antibody (Clone MABI 0302).

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me1 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3K4me1 antibody (mAb).

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 monomethyl Lys4 antibody (mAb) (Clone MABI 0302)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

This antibody is manufactured by MAB Institute, Inc.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K4me1 antibody (mAb)
 

Data Thumbnails

Histone H3K4me1 antibody (mAb) tested by ChIP.

ChIP of Histone H3K4me1 mAb.
(Click image to enlarge and see details.)

Histone H3K4me1 antibody (mAb) tested by Western blot.

Western blot of Histone H3K4me1 mAb.
(Click image to enlarge and see details.)

Histone H3K4me1 antibody (mAb) tested by dot blot analysis.

Dot blot of Histone H3K4me1 mAb.
(Click image to enlarge and see details.)