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Antibodies

Histone H3S10ph antibody (mAb)

Clone: MABI 0312
Catalog No: 39636 Format: 100 µg $410 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Application Notes


Validated Applications:
ICC/IF: 1 µg/ml dilution
WB: 0.2 - 2 µg/ml dilution

This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39253.

for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Immunogen

This Histone H3 phospho Ser10 antibody was raised against a peptide containing phospho serine 10 of human histone H3.

for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a PBS only version of this antibody is also available.

for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)References

 
Histone H3S10ph antibody (mAb) tested by immunofluorescence.

Histone H3 phospho Ser10 mAb tested by immunofluorescence.
Staining of HeLa cells with Histone H3 phospho Ser10 mAb (1 µg/ml, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

Histone H3S10ph antibody (mAb) tested by Western blot.

Histone H3S10ph antibody (mAb) tested by Western blot.
HeLa nuclear extract (30 µg per lane) was probed with Histone H3S10ph antibody at a 2 µg/ml dilution.
Lane 1: No treatment.
Lane 2: Cells treated with colcemid.

Histone H3S10ph antibody (mAb) tested by dot blot analysis.

Histone H3 phospho Ser10 mAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 mAb for phospho Ser10 histone H3. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: peptide phosphorylated at serine 10.
Lane 2: unmodified serine 10 peptide.
Lane 3: peptide phosphorylated at serine 28.
Lane 4: unmodified serine 28 peptide.

for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.

for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Positive Control

Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Ser10 antibody (Clone MABI 0312).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3S10ph antibody (mAb).

for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

This antibody is manufactured by MAB Institute, Inc.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3S10ph antibody (mAb)
 

Data Thumbnails

Histone H3S10ph antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3S10ph mAb.
(Click image to enlarge and see details.)

Histone H3S10ph antibody (mAb) tested by Western blot.

Western blot of Histone H3S10ph mAb.
(Click image to enlarge and see details.)

Histone H3S10ph antibody (mAb) tested by dot blot analysis.

Dot blot of Histone H3S10ph mAb.
(Click image to enlarge and see details.)