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Histone H3 phospho Ser10 antibody (mAb)
| Clone: MABI 0312 |
| Catalog No: 39636 | Format: 100 µg | $340 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| — | Not tested. ChIP-validated version available: 39253. |
1 µg/ml |
for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.
for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Immunogen
This antibody was raised against a peptide containing phospho serine 10 of human histone H3.
for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Application Notes
This Histone H3 phospho Ser10 antibody (Clone MABI 0312) (H3S10Ph) has been validated for use in immunofluorescence (1 µg/ml).
This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: 39253.
for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide.
for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Positive Control
Active Motif's HeLa acid extract (Paclitaxel treated) (Catalog No. 36201) can be used as a positive control for Histone H3 phospho Ser10 antibody (Clone MABI 0312).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 phospho Ser10 antibody (mAb).
for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)References
This antibody is described in Kimura, H. et al (2008) Cell Struct Func 33: 61-73.
Histone H3 phospho Ser10 mAb tested by immunofluorescence.
Staining of HeLa cells with Histone H3 phospho Ser10 mAb (1 µg/ml, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H3 phospho Ser10 mAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 mAb for phospho Ser10 histone H3. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: peptide phosphorylated at serine 10.
Lane 2: unmodified serine 10 peptide.
Lane 3: peptide phosphorylated at serine 28.
Lane 4: unmodified serine 28 peptide.
for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 phospho Ser10 antibody (mAb) (Clone MABI 0312)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
