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Histone H3 dimethyl Arg8 asymmetric antibody (pAb)
| Catalog No: 39651 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39652 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 | — |
for Histone H3 dimethyl Arg8 asymmetric antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.
for Histone H3 dimethyl Arg8 asymmetric antibody (pAb)Immunogen
This antibody was raised against a peptide containing dimethyl-Arg8 (asymmetric) of human histone H3.
for Histone H3 dimethyl Arg8 asymmetric antibody (pAb)Application Notes
This antibody has been validated for use in Western blotting (1:500 dilution).
for Histone H3 dimethyl Arg8 asymmetric antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Histone H3 dimethyl Arg8 asymmetric pAb tested by Western blot.
Nuclear extract from U2OS cells (20 µg) or HEK293 cells (20 µg) was probed with Histone H3 dimethyl Arg8 asymmetric pAb (1:500 dilution).
Histone H3 dimethyl Arg8 asymmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg8 asymmetric pAb for dimethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg8 asymmetric pAb at 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Top Panel:
Lane 1: unmodified Arg2 peptide.
Lane 2: monomethyl-Arg2 H3 peptide.
Lane 3: dimethyl-Arg2 H3 (symmetric) peptide.
Lane 4: dimethyl-Arg2 H3 (asymmetric) peptide.
Lane 5: unmodified Arg8 peptide.
Lane 6: monomethyl-Arg8 H3 peptide.
Lane 7: dimethyl-Arg8 H3 (symmetric) peptide.
Lane 8: dimethyl-Arg8 H3 (asymmetric) peptide.
Bottom Panel:
Lane 1: unmodified Arg17 H3 peptide.
Lane 2: monomethyl-Arg17 peptide.
Lane 3: dimethyl-Arg17 H3 (symmetric) peptide.
Lane 4: dimethyl-Arg17 H3 (asymmetric) peptide.
Lane 5: unmodified Arg26 peptide.
Lane 6: monomethyl-Arg26 H3 peptide.
Lane 7: dimethyl-Arg26 H3 (symmetric) peptide.
Lane 8: dimethyl-Arg26 H3 (asymmetric) peptide.
No detection of peptides (unmodified, mono-, di-, or tri-methylated) corresponding to lysine 4, lysine 9, lysine 18, lysine 23 and lysine 27 of Histone H3 was observed with 39651.
for Histone H3 dimethyl Arg8 asymmetric antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 dimethyl Arg8 asymmetric antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
