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Antibodies

Histone H3K23me2 antibody (pAb)

Catalog No: 39653 Format: 100 µl $410 Buy Now
Catalog No: 39654 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H3 dimethyl Lys23 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications:
ChIP
ICC/IF
See references for more information. Individual optimization may be required.

for Histone H3 dimethyl Lys23 antibody (pAb)Immunogen

This Histone H3 dimethyl Lys23 antibody was raised against a peptide containing dimethyl-Lys23 of human histone H3.

for Histone H3 dimethyl Lys23 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3 dimethyl Lys23 antibody (pAb)References

 
Histone H3K23me2 antibody (pAb) tested by Western blot.

Histone H3 dimethyl Lys23 pAb tested by Western blot.
HEK293 nuclear extract (25 µg) was probed with Histone Histone H3 dimethyl Lys23 pAb (1:500 dilution).

Histone H3K23me2 antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Lys23 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Lys23 pAb for dimethyl-Lys23 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with Histone H3 dimethyl Lys23 pAb at a dilution of 1: 2,000. The amount of peptide (in picomoles) spotted is indicated next to each row.
Top Panel:Lane 1: dimethyl Lys14. Lane 2: monomethyl Lys18. Lane 3: dimethyl Lys18. Lane 4: trimethyl Lys18. Lane 5: unmodified Lys23. Lane 6: monomethyl Lys23. Lane 7: dimethyl Lys23. Lane 8: trimethyl Lys23. Lane 9: unmodified Lys27. Lane 10: monomethyl Lys27. Lane 11: dimethyl Lys27. Lane 12: trimethyl Lys27. Bottom Panel: Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9.

for Histone H3 dimethyl Lys23 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure.Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.

for Histone H3 dimethyl Lys23 antibody (pAb)Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Histone H3K23me2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K23me2 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K23me2 antibody (pAb).

for Histone H3 dimethyl Lys23 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 dimethyl Lys23 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K23me2 antibody (pAb)
 

Data Thumbnails

Histone H3K23me2 antibody (pAb) tested by Western blot.

Western blot of Histone H3K23me2 pAb.
(Click image to enlarge and see details.)

Histone H3K23me2 antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K23me2 pAb.
(Click image to enlarge and see details.)