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Antibodies

HP1 gamma phospho Ser93 antibody (pAb)

Aliases: HECH, CBX3, HP1Hs-gamma
Catalog No: 39655 Format: 100 µl $410 Buy Now
Catalog No: 39656 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:25 kDa
Reactivity:Human

Applications

Western Blot Validated

for HP1 gamma phospho Ser93 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for HP1 gamma phospho Ser93 antibody (pAb)Immunogen

This HP1 gamma phospho Ser93 antibody was raised against a peptide containing phospho Ser93 of human HP1 gamma.

for HP1 gamma phospho Ser93 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for HP1 gamma phospho Ser93 antibody (pAb)References

 
HP1 gamma phospho Ser93 antibody (pAb) tested by Western blot.

HP1 gamma phospho Ser93 pAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with HP1 gamma phospho Ser93 pAb (1:500 dilution). Lane 1: no treatment. Lane 2: cells treated with colcemid.

HP1 gamma phospho Ser93 antibody (pAb) tested by dot blot analysis.

HP1 gamma phospho Ser93 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of HP1 gamma phospho Ser93 pAb for HP1 gamma phospho Ser93. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with HP1 gamma phospho Ser93 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.

Lane 1: unmodified HP1 gamma peptide.
Lane 2: phospho Ser93 HP1 gamma peptide.
Lane 3: phospho Ser92 HP1 alpha peptide.
Lane 4: phospho Ser89 HP1 beta peptide.

for HP1 gamma phospho Ser93 antibody (pAb)Background

Heterochromatin Protein 1 gamma (HP1 gamma) is one of the mammalian homologues of Drosophila HP1, first identified through its role in the maintenance of regions of the genome in a heterochromatic state. HP1 gamma contains a Chromo domain and a Chromo shadow domain. Through the Chromo domain, HP1 proteins are recruited to regions of chromatin that exhibit methylation of lysine 9 of histone H3. Phosphorylation of serine 93 of HP1 gamma increases during mitosis.

for HP1 gamma phospho Ser93 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HP1 gamma phospho Ser93 pAb.

for HP1 gamma phospho Ser93 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for HP1 gamma phospho Ser93 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

HP1 gamma phospho Ser93 antibody (pAb)
 

Data Thumbnails

HP1 gamma phospho Ser93 antibody (pAb) tested by Western blot.

Western blot of HP1 gamma phospho Ser93 pAb.
(Click image to enlarge and see details.)

HP1 gamma phospho Ser93 antibody (pAb) tested by dot blot analysis.

Dot blot of HP1 gamma phospho Ser93 pAb.
(Click image to enlarge and see details.)