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Antibodies

Aiolos antibody (mAb)

Clone: 8B2
Catalog No: 39657 Format: 100 µg $340 Buy Now
Catalog No: 39658 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein G Chromatography
Appl.:ChIP, WB
Reactivity:Mouse
Molecular Weight:70 kDa

Western Blot Chromatin IP
2 µg/ml 10 µl per ChIP

for Aiolos antibody (mAb) (Clone 8B2)Background

Aiolos (also known as IKZF3, Ikaros family zinc finger 3 and ZNFN1A3) belongs to a family of zinc-finger proteins that also include Ikaros and Helios. Aiolos is a transcription factor that is important in the regulation of lymphocyte proliferation and differentiation, which is preferentially expressed in peripheral blood leukocytes, spleen and thymus tissue. Both Ikaros and Aiolos can also participate in chromatin remodeling.

for Aiolos antibody (mAb) (Clone 8B2)Immunogen

This Aiolos antibody was raised against the N-terminal half of mouse Aiolos protein.

for Aiolos antibody (mAb) (Clone 8B2)Application Notes

This Aiolos antibody has been validated for use in Western blotting (2 µg/ml dilution) and ChIP (10 µl per ChIP).

for Aiolos antibody (mAb) (Clone 8B2)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for Aiolos antibody (mAb) (Clone 8B2)Positive Control

This antibody can be used with lymphocytes, spleen, thymus or bone marrow samples.

Chromatin IP Kits & Reagents

Active Motif has a variety of Chromatin Immunoprecipitation kits and reagents for every need. Please visit our Chromatin IP Page to learn about our ChIP kits, controls, chromatin and PCR primers for use with Aiolos antibody (mAb).

Bridging Antibody to Improve ChIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP and IP experiments that use Aiolos antibody (mAb).

for Aiolos antibody (mAb) (Clone 8B2)References

Thompson, E.C. et al. (2007) Immunity. PMID: 17363301

for Aiolos antibody (mAb) (Clone 8B2)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Aiolos antibody (mAb) (Clone 8B2)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

Aiolos antibody (mAb)