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Antibodies

MAD1 antibody (mAb)

Clone: 9B10
Catalog No: 39659 Format: 100 µg $340 Buy Now
Catalog No: 39660 Format: 10 µg $70 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein G Chromatography
Applications:IF, WB
Reactivity:Human, Mouse
Molecular Weight:85 kDa

Applications Table

Western Blot Chromatin IP Immunofluorescence
5 µg/ml — 2 µg/ml

for MAD1 antibody (mAb) (Clone 9B10)Background

MAD1 (MAD1L1, Mitotic arrest deficient 1) is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD1 homoderizes with MAD2L1 and is present at the kinetochore. Defects in MAD1 are involved in the development and/or progression of various types of cancer.

for MAD1 antibody (mAb) (Clone 9B10)Immunogen

This antibody was raised against a peptide corresponding to amino acids 331-718 of mouse MAD1.

for MAD1 antibody (mAb) (Clone 9B10)Application Notes

This antibody has been validated for use in immunofluorescence (2 µg/ml) and Western blotting (5 µg/ml dilution).

for MAD1 antibody (mAb) (Clone 9B10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MAD1 antibody (mAb).

for MAD1 antibody (mAb) (Clone 9B10)References

Liu S et al 2003. Nature Cell Biol 5: 341-345.
Liu S et al 2006. J Cell Biol 175: 41-53.
Feng J et al 2006. Chromosoma 115: 320-329.
Huang H et al 2007. J Cell Biol 177:413-424.

for MAD1 antibody (mAb) (Clone 9B10)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for MAD1 antibody (mAb) (Clone 9B10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting