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Phc2 antibody (mAb)
| Catalog No: 39661 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39662 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| — | Yes | Yes | Yes |
for Phc2 antibody (mAb)Background
Phc2, polyhomeotic homolog 2 (also known as EDR2, early development regulatory protein 2) is a component of the polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is required to maintain the stable repression of the Hox genes during development. This chromatin-associated protein complex acts via chromatin remodeling and histone modifications. Phc2 has been shown to interact with PHC1, BMI1, MAPKAPK2 and MCRS1.
for Phc2 antibody (mAb)Immunogen
This Phc2 antibody was raised against a recombinant fragment of human Phc2.
for Phc2 antibody (mAb)Application Notes
This Phc2 antibody has been validated for use in the following applications:
ChIP: see reference 1
IF: see reference 2
IP: see reference 1
for Phc2 antibody (mAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Phc2 antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Phc2 antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Phc2 antibody (mAb).
for Phc2 antibody (mAb)References
1. Isono, K. et. al. (2005) Mol. Cell. Bio. 25: 6694-6706.
2. Takada, Y. et. al. (2007) Development 134: 579-590.
for Phc2 antibody (mAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Phc2 antibody (mAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
