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Histone H3 monomethyl Arg8 antibody (pAb)
| Catalog No: 39673 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39674 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for Histone H3 monomethyl Arg8 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.
for Histone H3 monomethyl Arg8 antibody (pAb)Immunogen
This antibody was raised against a peptide containing monomethyl-Arg8 of human histone H3.
for Histone H3 monomethyl Arg8 antibody (pAb)Application Notes
This product has been validated for use in Western blotting (1:500 - 1:2,000 dilution).
for Histone H3 monomethyl Arg8 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
Histone H3 monomethyl Arg8 pAb tested by Western blot.
HeLa cell nuclear extract (20 µg) probed with Histone H3 monomethyl Arg8 pAb (1:500 dilution).
Histone H3 monomethyl Arg8 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Arg8 pAb for monomethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 monomethyl Arg8 pAb at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Top Panel:
Lane 1: unmodified Arg2 peptide.
Lane 2: monomethyl-Arg2 H3 peptide.
Lane 3: dimethyl-Arg2 H3 (symmetric) peptide.
Lane 4: dimethyl-Arg2 H3 (asymmetric) peptide.
Lane 5: unmodified Arg8 peptide.
Lane 6: monomethyl-Arg8 H3 peptide.
Lane 7: dimethyl-Arg8 H3 (symmetric) peptide.
Lane 8: dimethyl-Arg8 H3 (asymmetric) peptide.
Bottom Panel:
Lane 1: unmodified Arg17 H3 peptide.
Lane 2: monomethyl-Arg17 peptide.
Lane 3: dimethyl-Arg17 H3 (symmetric) peptide.
Lane 4: dimethyl-Arg17 H3 (asymmetric) peptide.
Lane 5: unmodified Arg26 peptide.
Lane 6: monomethyl-Arg26 H3 peptide.
Lane 7: dimethyl-Arg26 H3 (symmetric) peptide.
Lane 8: dimethyl-Arg26 H3 (asymmetric) peptide.
for Histone H3 monomethyl Arg8 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 monomethyl Arg8 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
