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Histone H3 acetyl Lys27 antibody (mAb)
| Clone: MABI 0309 |
| Catalog No: 39685 | Format: 100 µg | $340 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 0.5 - 2 µg/ml | Published. See Application Notes below. |
1 - 2 µg/ml |
for Histone H3 acetyl Lys27 antibody (mAb) (Clone MABI 0309)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation.
Lysine 27 of histone H3 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.
for Histone H3 acetyl Lys27 antibody (mAb) (Clone MABI 0309)Immunogen
This antibody was raised against a synthetic peptide including acetyl-lysine 27 of human histone H3.
for Histone H3 acetyl Lys27 antibody (mAb) (Clone MABI 0309)Application Notes
This antibody has been validated for use in Western blotting (0.5 - 2 µg / ml dilution), immunofluorescence (1 - 2 µg / ml dilution) and reported to work in chromatin IP (1 - 10 µg per ChIP; see Reference "Kimura et al, 2008").
for Histone H3 acetyl Lys27 antibody (mAb) (Clone MABI 0309)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide.
for Histone H3 acetyl Lys27 antibody (mAb) (Clone MABI 0309)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys27 mAb (Clone MABI 0309).
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 acetyl Lys27 antibody (mAb).
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Histone H3 acetyl Lys27 antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 acetyl Lys27 antibody (mAb).
for Histone H3 acetyl Lys27 antibody (mAb) (Clone MABI 0309)References
This antibody was used in ChIP assays as described in Kimura, H. et al (2008) Cell Struct Func 33: 61-73.
Histone H3 acetyl Lys27 mAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys27 mAb (2 µg/ml dilution).
Lane 1: No treatment.
Lane 2: cells treated with sodium butyrate.
Histone H3 acetyl Lys27 mAb tested by immunofluorescence.
Staining of HeLa cells with Histone H3 acetyl Lys27 mAb (1 µg/ml, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).
Histone H3 acetyl Lys27 mAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys27 mAb for acetyl-Lys27 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 0.2 µg/ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys4 peptide.
Lane 2: acetyl-Lys9 peptide.
Lane 3: acetyl-Lys14 peptide.
Lane 4: acetyl-Lys18 peptide.
Lane 5: acetyl-Lys23 peptide.
Lane 6: acetyl-Lys27 peptide.
Lane 7: acetyl-Lys36 peptide.
Lane 8: acetyl-Lys37 peptide.
Lane 9: acetyl-Lys64 peptide.
Lane 10: acetyl-Lys79 peptide.
for Histone H3 acetyl Lys27 antibody (mAb) (Clone MABI 0309)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 acetyl Lys27 antibody (mAb) (Clone MABI 0309)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
