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Antibodies

Histone H3K18ac antibody (pAb)

Catalog No: 39693 Format: 100 µl $410 Buy Now
Catalog No: 39694 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunocytochemistry Validated

for Histone H3 acetyl Lys18 antibody (pAb)Application Notes


Validated Applications:
ICC/IF: 1:100 - 1:500 dilution
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP
WB

See references for more information. Individual optimization may be required.

for Histone H3 acetyl Lys18 antibody (pAb)Immunogen

This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3.

for Histone H3 acetyl Lys18 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3 acetyl Lys18 antibody (pAb)References

 
Histone H3K18ac antibody (pAb) tested by immunofluorescence.

Histone H3 acetyl Lys18 pAb tested by immunofluorescence.
Left: HeLa cells stained with Histone H3 acetyl Lys18 pAb. Right: DAPI stain.

Histone H3K18ac antibody (pAb) tested by Western blot.

Histone H3 acetyl Lys18 pAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys18 pAb (1:2,000 dilution).
Lane 1: Untreated cells.
Lane 2: Cells treated with sodium butyrate.

Histone H3K18ac antibody (pAb) tested by dot blot analysis.

Histone H3 acetyl Lys18 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 pAb for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:500. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys4 peptide. Lane 2: acetyl-Lys9 peptide. Lane 3: acetyl-Lys14 peptide. Lane 4: acetyl-Lys18 peptide. Lane 5: acetyl-Lys23 peptide. Lane 6: acetyl-Lys27 peptide. Lane 7: acetyl-Lys36 peptide. Lane 8: acetyl-Lys37 peptide. Lane 9: acetyl-Lys64 peptide. Lane 10: acetyl-Lys79 peptide.

for Histone H3 acetyl Lys18 antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.

for Histone H3 acetyl Lys18 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3K18ac antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K18ac antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K18ac antibody (pAb).

for Histone H3 acetyl Lys18 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 acetyl Lys18 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3K18ac antibody (pAb)
 

Data Thumbnails

Histone H3K18ac antibody (pAb) tested by immunofluorescence.

Immunofluorescence stain of Histone H3K18ac pAb.
(Click image to enlarge and see details.)

Histone H3K18ac antibody (pAb) tested by Western blot.

Western blot of Histone H3K18ac pAb.
(Click image to enlarge and see details.)

Histone H3K18ac antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3K18ac pAb.
(Click image to enlarge and see details.)