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Histone H3 acetyl Lys18 antibody (pAb)
| Catalog No: 39693 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39694 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunofluorescence |
|---|---|---|
| 1:500 - 1:2,000 | Published. See Application Notes below. |
1:100 - 1:500 |
for Histone H3 acetyl Lys18 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.
for Histone H3 acetyl Lys18 antibody (pAb)Immunogen
This antibody was raised against a peptide including acetyl-lysine 18 of histone H3.
for Histone H3 acetyl Lys18 antibody (pAb)Application Notes
This antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution) and immunofluorescence (1:100 - 1:500 dilution).
It has also been reported to work in chromatin immunoporecipitation (ChIP) using 4 µl per ChIP on chromatin derived from 5 x 10^6 cells (see Reference Ferrari et al, 2008).
for Histone H3 acetyl Lys18 antibody (pAb)Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide.
for Histone H3 acetyl Lys18 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 acetyl Lys18 antibody (pAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3 acetyl Lys18 antibody (pAb).
for Histone H3 acetyl Lys18 antibody (pAb)References
This antibody was used in ChIP assays as described in Ferrari, R. et al (2008) Science 321: 1086-1088.
Histone H3 acetyl Lys18 pAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H3 acetyl Lys18 pAb (1:2,000 dilution).
Lane 1: Untreated cells.
Lane 2: Cells treated with sodium butyrate.
Histone H3 acetyl Lys18 pAb tested by immunofluorescence.
Left: HeLa cells stained with Histone H3 acetyl Lys18 pAb. Right: DAPI stain.
Histone H3 acetyl Lys18 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys18 pAb for acetyl Lys18 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:500. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys4 peptide. .
Lane 2: acetyl-Lys9 peptide. .
Lane 3: acetyl-Lys14 peptide.
Lane 4: acetyl-Lys18 peptide.
Lane 5: acetyl-Lys23 peptide.
Lane 6: acetyl-Lys27 peptide.
Lane 7: acetyl-Lys36 peptide.
Lane 8: acetyl-Lys37 peptide.
Lane 9: acetyl-Lys64 peptide.
Lane 10: acetyl-Lys79 peptide.
for Histone H3 acetyl Lys18 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 acetyl Lys18 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
