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Mi-2 beta antibody (mAb)
| Clone: 3F2/4 |
| Catalog No: 39695 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39696 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | Immunoprecipitation |
|---|---|---|
| 1 - 2 µg/ml | — | Yes |
for Mi-2 beta antibody (mAb) (Clone 3F2/4)Background
Mi-2β (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).
for Mi-2 beta antibody (mAb) (Clone 3F2/4)Immunogen
This antibody was raised against a synthetic peptide derived from human Mi-2B.
for Mi-2 beta antibody (mAb) (Clone 3F2/4)Application Notes
This antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution) and has been reported to work in immunoprecipitation.
for Mi-2 beta antibody (mAb) (Clone 3F2/4)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
for Mi-2 beta antibody (mAb) (Clone 3F2/4)Positive Control
Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for AML-1/Runx1 pAb.
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Mi-2 beta antibody (mAb).
Mi-2 beta pAb tested by Western blot.
Nuclear extract of Jurkat cells stained with Mi-2 beta mAb at a dilution of 2 µg/ml.
for Mi-2 beta antibody (mAb) (Clone 3F2/4)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Mi-2 beta antibody (mAb) (Clone 3F2/4)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
