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Histone H3 acetyl Lys14 antibody (pAb)
| Catalog No: 39697 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39698 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 0.5 - 2 µg/ml | 2 - 5 µg per ChIP |
for Histone H3 acetyl Lys14 antibody (pAb)Background
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.
for Histone H3 acetyl Lys14 antibody (pAb)Immunogen
This antibody was raised against a peptide including acetyl-lysine 14 of histone H3.
for Histone H3 acetyl Lys14 antibody (pAb)Application Notes
This antibody has been validated for use in chromatin IP (2 - 5 µg per ChIP) and in Western blotting (0.5 - 2 µg/ml dilution).
At high antibody titre, slight detection of acetylation at lysine 23 of histone H3 is observed by dot blot analysis.
for Histone H3 acetyl Lys14 antibody (pAb)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide.
for Histone H3 acetyl Lys14 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys14 pAb.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3 acetyl Lys14 antibody (pAb).
Histone H3 acetyl Lys14 pAb tested by Western blot.
HeLa acid extract (10 µg per lane) was probed with Histone H3 acetyl Lys14 pAb (1:500 dilution).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate.
Histone H3 acetyl Lys14 pAb tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 5 µg of Histone H3 acetyl Lys14 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H3 acetyl Lys14 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys14 pAb for acetyl Lys14 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 µg per ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys9 peptide.
Lane 2: unmodified Lys9 peptide.
Lane 3: acetyl-Lys14 peptide.
Lane 4: unmodified Lys14 peptide.
Lane 5: acetyl-Lys18 peptide.
Lane 6: acetyl-Lys23 peptide.
Lane 7: acetyl-Lys27 peptide.
No detection of peptides acetylated at either lysine 4, 36, 37, 64 or 79 of histone H3 was observed (data not shown).
for Histone H3 acetyl Lys14 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H3 acetyl Lys14 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
