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Histone H4 acetyl Lys5 antibody (pAb)
| Catalog No: 39699 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39700 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 0.5 - 2 µg/ml | 5 µg per ChIP |
for Histone H4 acetyl Lys5 antibody (pAb)Background
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. H4 molecules acetylated at Lys5 or Lys8 are distributed in overlapping, but non-identical, islands throughout the euchromatic chromosome arms.
for Histone H4 acetyl Lys5 antibody (pAb)Immunogen
This antibody was raised against a peptide including acetyl-lysine 5 of human histone H4.
for Histone H4 acetyl Lys5 antibody (pAb)Application Notes
This antibody has been validated for use in Western blotting (0.5 - 2 µg/ml dilution) and chromatin IP (5 µg per ChIP).
for Histone H4 acetyl Lys5 antibody (pAb)Buffer
PBS pH 7.5, 30% glycerol, 0.035% sodium azide.
for Histone H4 acetyl Lys5 antibody (pAb)Positive Control
Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H4 acetyl Lys5 pAb.
Chromatin IP Assays
Active Motif has a variety of Chromatin Immunoprecipitation Kits for every need.
Please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H4 acetyl Lys5 antibody (pAb).
Histone H4 acetyl Lys5 pAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) probed with Histone H4 acetyl Lys5 polyclonal antibody (0.5 µg per ml).
Lane 1: No treatment.
Lane 2: Cells treated with sodium butyrate.
Histone H4 acetyl Lys5 pAb tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 5 µg of Histone H4 acetyl Lys5 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
Histone H4 acetyl Lys5 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H4 acetyl Lys5 pAb for acetyl Lys5 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 µg per ml. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: acetyl-Lys5 peptide.
Lane 2: unmodified Lys5 peptide.
Lane 3: acetyl-Lys8 peptide.
Lane 4: unmodified Lys8 peptide.
Lane 5: acetyl-Lys12 peptide.
Lane 6: unmodified Lys12 peptide.
Lane 7: acetyl-Lys16 peptide.
Lane 8: unmodified Lys16 peptide.
for Histone H4 acetyl Lys5 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Histone H4 acetyl Lys5 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
