Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

Histone H3R17me2a (asymmetric) antibody (pAb)

Catalog No: 39709 Format: 100 µl $410 Buy Now
Catalog No: 39710 Format: 10 µl $95 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human, Other (Wide Range)

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated Immunohistochemistry Validated

for Histone H3 dimethyl Arg17 asymmetric antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP
WB
IHC
IF

for Histone H3 dimethyl Arg17 asymmetric antibody (pAb)Immunogen

This Histone H3 dimethyl Arg17 asymmetric antibody was raised against a peptide containing dimethyl-Arg17 (asymmetric) of human histone H3.

for Histone H3 dimethyl Arg17 asymmetric antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Histone H3 dimethyl Arg17 asymmetric antibody (pAb)References

 
Histone H3R17me2a (asymmetric) antibody (pAb) tested by Western blot.

Histone H3 dimethyl Arg17 asymmetric antibody (pAb) tested by Western blot.
HeLa nuclear extract (25 µg) probed with Histone H3 dimethyl Arg17 asymmetric antibody at a 1:500 dilution.

Histone H3R17me2a (asymmetric) antibody (pAb) tested by dot blot analysis.

Histone H3 dimethyl Arg17 asymmetric pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 dimethyl Arg17 asymmetric pAb for dimethyl Arg17 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 dimethyl Arg17 asymmetric pAb at a dilution of 1:30,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Top panel Lane 1: unmodified Arg2. Lane 2: monomethyl-Arg2 H3. Lane 3: dimethyl-Arg2 H3 (sym). Lane 4: dimethyl-Arg2 H3 (asym). Lane 5: unmodified Arg8. Lane 6: monomethyl-Arg8 H3. Lane 7: dimethyl-Arg8 H3 (sym). Lane 8: dimethyl-Arg8 H3 (asym).
Bottom panel Lane 1: unmodified Arg17. Lane 2: monomethyl-Arg17 H3. Lane 3: dimethyl-Arg17 H3 (asym). Lane 4: dimethyl-Arg17 H3 (sym). Lane 5: unmodified Arg26. Lane 6: monomethyl-Arg26 H3. Lane 7: dimethyl-Arg26 H3 (asym). Lane 8: dimethyl-Arg26 H3 (sym).

for Histone H3 dimethyl Arg17 asymmetric antibody (pAb)Background

Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine.

for Histone H3 dimethyl Arg17 asymmetric antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 dimethyl Arg17 asymmetric pAb.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3R17me2a (asymmetric) antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3R17me2a (asymmetric) antibody (pAb).

for Histone H3 dimethyl Arg17 asymmetric antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Histone H3 dimethyl Arg17 asymmetric antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Histone H3R17me2a (asymmetric) antibody (pAb)
 

Data Thumbnails

Histone H3R17me2a (asymmetric) antibody (pAb) tested by Western blot.

Western blot of Histone H3R17me2a (asymmetric) pAb.
(Click image to enlarge and see details.)

Histone H3R17me2a (asymmetric) antibody (pAb) tested by dot blot analysis.

Dot blot of Histone H3R17me2a (asymmetric) pAb.
(Click image to enlarge and see details.)