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Antibodies

PHF8 antibody (pAb)

Aliases: JHDM1F, MRXSSD, ZNF422
Catalog No: 39711 Format: 100 µl $410 Buy Now
Catalog No: 39712 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:135 kDa
Reactivity:Human

Applications

Western Blot Validated

for PHF8 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Validated Applications:
WB

for PHF8 antibody (pAb)Immunogen

This PHF8 antibody was raised against a synthetic peptide derived from human PHF8.

for PHF8 antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PHF8 antibody (pAb)References

 
PHF8 antibody (pAb) tested by Western blot.

PHF8 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with PHF8 pAb (1:500 dilution).

for PHF8 antibody (pAb)Background

PHF8 (PHD finger protein 8) is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. PHF8 also has an N-terminal PHD finger and demethylates Histone H3 monomethyl Lys9 (H3K9me1) and Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2) (which are all modifications associated with transcriptional repression) and also Histone H3 dimethyl Lys36 (H3K36me2). Defects in PHF8 lead to Siderius type X-linked mental retardation (MRXSSD).

for PHF8 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for PHF8 pAb.

for PHF8 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PHF8 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PHF8 antibody (pAb)
 

Data Thumbnails

PHF8 antibody (pAb) tested by Western blot.

Western blot of PHF8 pAb.
(Click image to enlarge and see details.)