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Antibodies

CID antibody (pAb)

Aliases: CenH3, cid, BcDNA:RE21270, cenH3, CenH3/CID, CenH3[Cid], CenH3[CID], Cenp-A, CENP-A, CENP-A/Cid, CENP-A/CID, CENP-C, cenpA, CenpA, CENPA, CenpA/CID, CG13329, Cid, CID/CENP-A, Cid[Mel], Dmel\CG13329
Catalog No: 39713 Format: 100 µl $410 Buy Now
Catalog No: 39714 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:Serum
Purification:None
Host:Rabbit
Molecular Weight:30 kDa
Reactivity:Drosophila

Applications

Western Blot Validated

for CID antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 dilution

For an antibody against CID that works in immunofluorescence, use Catalog No. 39719.

for CID antibody (pAb)Immunogen

This CID antibody was raised against recombinant CID protein from Drosophila melanogaster.

for CID antibody (pAb)Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for CID antibody (pAb)References

 
CID antibody (pAb) tested by Western blot.

CID pAb tested by Western blot.
DrosophilaSchneider L2 cells nuclear extract (10 µg) probed with CID pAb (1:500 dilution).

for CID antibody (pAb)Background

CID (Centromer Identifier) is the Drosophila histone H3 variant which replaces canonical H3 in centromeric chromatin at the inner plate of the kinetochore (similar to mammalian CENP-A). CID is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. It may serve as an epigenetic mark that propagates centromere identity through DNA replication and cell division.

for CID antibody (pAb)Positive Control

Active Motif's Schneider's Drosophila L2 nuclear extract (Catalog No. 36087) can be used as a positive control for CID antibody.

for CID antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for CID antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

CID antibody (pAb)
 

Data Thumbnails

CID antibody (pAb) tested by Western blot.

Western blot of CID pAb.
(Click image to enlarge and see details.)