CID antibody (pAb)

RRID: AB_2793317
Aliases: CenH3, cid, BcDNA:RE21270, cenH3, CenH3/CID, CenH3[Cid], CenH3[CID], Cenp-A, CENP-A, CENP-A/Cid, CENP-A/CID, CENP-C, cenpA, CenpA, CENPA, CenpA/CID, CG13329, Cid, CID/CENP-A, Cid[Mel], Dmel\CG13329
Catalog No: 39713 Format: 100 µl Discontinued
Catalog No: 39714 Format: 10 µl Discontinued

Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
30 kDa
Reactivity:
Drosophila

Applications

Western Blot Validated

Application Notes

This product has been discontinued
Validated Applications:
WB: 1:500 dilution

Published Applications:
WB

For an antibody against CID that works in immunofluorescence, use Catalog No. 39719.

Immunogen

This CID antibody was raised against recombinant CID protein from Drosophila melanogaster.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

CID pAb tested by Western blot.
DrosophilaSchneider L2 cells nuclear extract (10 µg) probed with CID pAb (1:500 dilution).

Background

CID (Centromer Identifier) is the Drosophila histone H3 variant which replaces canonical H3 in centromeric chromatin at the inner plate of the kinetochore (similar to mammalian CENP-A). CID is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. It may serve as an epigenetic mark that propagates centromere identity through DNA replication and cell division.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting