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Antibodies

CID antibody (pAb)

Catalog No: 39713 Format: 100 µl $340 Buy Now
Catalog No: 39714 Format: 10 µl $70 Buy Now
Antibody Type:Polyclonal
Host:Rabbit
Isotype:Serum
Purification:None
Applications:WB
Reactivity:Drosophila
Molecular Weight:30 kDa

Applications Table

Western Blot Chromatin IP
1:500

for CID antibody (pAb)Background

CID (Centromer Identifier) is the Drosophila histone H3 variant which replaces canonical H3 in centromeric chromatin at the inner plate of the kinetochore (similar to mammalian CENP-A). CID is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. It may serve as an epigenetic mark that propagates centromere identity through DNA replication and cell division.

for CID antibody (pAb)Immunogen

This antibody was raised against recombinant CID protein from Drosophila melanogaster.

for CID antibody (pAb)Application Notes

This antibody has been validated for use in Western blot (1:500 dilution). For an antibody against CID that works in immunofluorescence, use Catalog No. 39719.

for CID antibody (pAb)Buffer

Rabbit serum containing 30% glycerol, 0.035% sodium azide

 
CID pAb tested by Western blot.

CID pAb tested by Western blot.
DrosophilaSchneider L2 cells nuclear extract (10 µg) probed with CID pAb (1:500 dilution).

for CID antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for CID antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting
 

Technical Data Sheet

CID antibody (pAb)
 

Data Thumbnails

CID pAb tested by Western blot.

Western blot of CID pAb.
(Click image to enlarge and see details).