HDAC3 antibody (mAb)

for HDAC3 antibody (mAb) (Clone 3G6)Background

HDAC3 (Histone Deacetylase 3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

By forming multi-protein complexes with the co-repressors SMRT and N-CoR, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.

for HDAC3 antibody (mAb) (Clone 3G6)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acids 411-428 of human HDAC3.

for HDAC3 antibody (mAb) (Clone 3G6)Application Notes

This antibody is reported to have been validated for use in immunoprecipitation (2-5 µg per IP), Western blotting and immunohistochemistry. This antibody can be used to immunoprecipitate HDAC3 activity as well as the protein itself. See references for additional information.

for HDAC3 antibody (mAb) (Clone 3G6)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for HDAC3 antibody (mAb) (Clone 3G6)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HDAC3 mAb (Clone 3G6).

for HDAC3 antibody (mAb) (Clone 3G6)References

This antibody has been used in Nevels, M. et al (2004) PNAS USA 101: 17234-17239. and Li, Y. et al (2006) Genes Dev 20: 2566-2579.

for HDAC3 antibody (mAb) (Clone 3G6)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for HDAC3 antibody (mAb) (Clone 3G6)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.