Acf1 antibody (pAb)

for Acf1 antibody (pAb)Background

Drosophila Acf1 is a component of ACF, the ATP-utilizing chromatin assembly and remodeling factor protein complex. ACF catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and ISWI. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex.

for Acf1 antibody (pAb)Immunogen

This antibody was raised against recombinant C-terminal fragment of Acf1 from Drosophila melanogaster.

for Acf1 antibody (pAb)Application Notes

This antibody has been validated for use in Western blot (1:500 - 1:1,000), immunofluorescence (1:1,000) and immunoprecipitation (10 µl per IP).
This antibody was used in Fyodorov et al, 2004.

Native Acf1 is expressed as two isoforms with apparent molecular masses of 185 and 170 kDa. The 170-kDa isoform is produced by proteolytic clipping of the C-terminus from the full-length 185-kDa polypeptide. Therefore this antibody recognizes primarily the 185-kDa isoform.

Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for Acf1 antibody (pAb)Buffer

PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol.

for Acf1 antibody (pAb)References

Fyodorov D. et al (2004) Genes Dev 18: 170–183.

for Acf1 antibody (pAb)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Acf1 antibody (pAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.