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Phc1 antibody (mAb)
| Clone: 6-1-3 |
| Catalog No: 39723 | Format: 100 µg | $340 | Buy Now |
| Catalog No: 39724 | Format: 10 µg | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP | IF | IP |
|---|---|---|---|
| Yes | — | Yes | Yes |
for Phc1 antibody (mAb) (Clone 6-1-3)Background
Phc1, polyhomeotic homolog 1 (also known as EDR1, early development regulatory protein 1) is a component of the polycomb group (PcG) multiprotein PRC1 complex. The PRC1 complex is required to maintain the stable repression of the Hox genes during development. This chromatin-associated protein complex acts via chromatin remodeling and histone modifications. Phc1 has been shown to interact with PHC2, BMI1, MAPKAPK2 and MCRS1.
for Phc1 antibody (mAb) (Clone 6-1-3)Immunogen
This antibody was raised against full length recombinant mouse Phc1 protein.
for Phc1 antibody (mAb) (Clone 6-1-3)Application Notes
This antibody has been validated for use in Western blot, immunofluorescence and immunoprecipitation. The usage of this antibidy is described in references Miyagishima et al, 2003 and Osono et al, 2005.
for Phc1 antibody (mAb) (Clone 6-1-3)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Bridging Antibody to Improve ChIP, MeDIP and IP
Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Phc1 antibody (mAb).
Compatible Secondary Antibodies
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Phc1 antibody (mAb).
for Phc1 antibody (mAb) (Clone 6-1-3)References
1. Miyagishima et al (2003) Histochem Cell Biol 120: 111-119.
2. Isono, K. et. al. (2005) Mol. Cell. Bio. 25: 6694-6706.
for Phc1 antibody (mAb) (Clone 6-1-3)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Phc1 antibody (mAb) (Clone 6-1-3)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
