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Ubiquitin antibody (mAb)

Clone: P4D1
Catalog No: 39741 Format: 100 µg $425 Buy Now
Catalog No: 39742 Format: 10 µg $99 Buy Now

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Antibody Type:Monoclonal
Purification:Protein G Chromatography
Reactivity:Other (Wide Range)


Western Blot Validated

for Ubiquitin antibody (mAb) (Clone P4D1)Application Notes

Published Applications:

For additional information, please see the original publication for the generation of this clone: Kahana, A., and Gottschling, D.E., Mol. Cell. Biol. 19: 6608-6620, 1999.

for Ubiquitin antibody (mAb) (Clone P4D1)Immunogen

This Ubiquitin antibody was raised against purified bovine ubiquitin.

for Ubiquitin antibody (mAb) (Clone P4D1)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Ubiquitin antibody (mAb) (Clone P4D1)References

for Ubiquitin antibody (mAb) (Clone P4D1)Background

Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.

for Ubiquitin antibody (mAb) (Clone P4D1)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Ubiquitin antibody (mAb) (Clone P4D1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Ubiquitin antibody (mAb)