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Antibodies

Ubiquitin antibody (mAb)

Clone: P4D1
Catalog No: 39741 Format: 100 µg $370 Buy Now
Catalog No: 39742 Format: 10 µg $80 Buy Now
Antibody Type:Monoclonal
Host:Mouse
Isotype:IgG
Purification:Protein G Chromatography
Applications:IF, IHC, IP, WB
Reactivity:Other (Wide Range)

for Ubiquitin antibody (mAb) (Clone P4D1)Immunogen

This Ubiquitin antibody was raised against purified bovine ubiquitin.

for Ubiquitin antibody (mAb) (Clone P4D1)Application Notes

This Ubiquitin antibody was validated for use in Western blotting, immunofluorescence and immunoprecipitation. The usage of this antibody is described in Kahana and Gottschling, 1999.

for Ubiquitin antibody (mAb) (Clone P4D1)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Ubiquitin antibody (mAb) (Clone P4D1)References

Kahana, A and D.E. Gottschling, 1999. Mol Cell Biol 19: 6608-6620.

for Ubiquitin antibody (mAb) (Clone P4D1)Background

Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Ubiquitin antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Ubiquitin antibody (mAb).

for Ubiquitin antibody (mAb) (Clone P4D1)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Ubiquitin antibody (mAb) (Clone P4D1)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FACS = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Ubiquitin antibody (mAb)