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Antibodies

PARP-2 antibody (pAb)

Aliases: ARTD2, ADPRT2, ADPRTL2, ADPRTL3, pADPRT-2
Catalog No: 39743 Format: 100 µl $410 Buy Now
Catalog No: 39744 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:66 kDa
Reactivity:Human

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated

for PARP-2 antibody (pAb)Application Notes


Validated Applications:
ChIP: 2 - 10 µl per ChIP
IP: 2 - 10 µl per IP
WB: 1:500 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Validated Applications:
WB
IF

for PARP-2 antibody (pAb)Immunogen

This PARP-2 antibody was raised against a recombinant protein corresponding to the C-terminal region of human PARP-2.

for PARP-2 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for PARP-2 antibody (pAb)References

 
PARP-2 antibody (pAb) tested by ChIP.

PARP-2 antibody tested by ChIP analysis.
Chromatin IP performed on stably transfected AC16 cells using the per-immune serum (Pre) or the PARP-2 antibody. ChIP DNA was subjected to qPCR using primers specific for human CD36 gene. Data are presented relative to input.

PARP-2 antibody (pAb) tested by immunoprecipitation.

PARP-2 antibody used for immunoprecipitation.
Western blot of immunoprecipitation using the PARP-2 antibody. Lane 1: input (AC16 whole cell lystate). Lane 2: IP using pre-immune serum. Lane 3: IP using PARP-2 antibody.

PARP-2 antibody (pAb) tested by Western blot.

PARP-2 antibody tested by Western blot.
Lane 1: U2OS nuclear extract (20 µg). Lane 2: A549 whole-cell extract(20 µg). Lane 3: Hep G2 whole-cell extract (20 µg). Lane 4: Jurkat cell nuclear extract (20 µg). These were probed with PARP-2 antibody at a dilution of 1:500.

for PARP-2 antibody (pAb)Background

PARP-2 encodes a chromatin-associated enzyme, a poly-ADP ribosyl transferase, which modifies various nuclear proteins by poly-ADP ribosylation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. PARP-2 is cleaved by caspase enzymes during the process of apoptosis.

for PARP-2 antibody (pAb)Positive Control

Active Motif's Hep G2 whole-cell extract (Catalog No. 36412) can be used as a positive control for PARP-2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PARP-2 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with PARP-2 antibody (pAb).

for PARP-2 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for PARP-2 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

PARP-2 antibody (pAb)
 

Data Thumbnails

PARP-2 antibody (pAb) tested by ChIP.

ChIP of PARP-2 pAb.
(Click image to enlarge and see details.)

PARP-2 antibody (pAb) tested by immunoprecipitation.

Immunoprecipitation of PARP-2 pAb.
(Click image to enlarge and see details.)

PARP-2 antibody (pAb) tested by Western blot.

Western blot of PARP-2 pAb.
(Click image to enlarge and see details.)