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Antibodies

E1A antibody (mAb)

Clone: M73
Catalog No: 39771 Format: 100 µg $410 Buy Now
Catalog No: 39772 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Mouse
Reactivity:Other (Not Species Specific)

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

for E1A antibody (mAb) (Clone M73)Application Notes


Validated Applications:
WB: 0.1 - 1 µg/ml dilution

Published Applications:
IP
ICC/IF
See references for more information. Individual optimization may be required.

for E1A antibody (mAb) (Clone M73)Immunogen

This E1A antibody was raised against a recombinant protein corresponding to full-length E1A from Adenovirus 2.

for E1A antibody (mAb) (Clone M73)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for E1A antibody (mAb) (Clone M73)References

 
E1A antibody (mAb) tested by Western blot.

E1A antibody tested by Western blot.
HEK293 whole-cell extract probed with E1A antibody (Clone M73) (0.1 μg/ml dilution). Arrows indicate the Ad2 and Ad5 E1A gene products.

for E1A antibody (mAb) (Clone M73)Background

Adenovirus E1A protein is a transcriptional activator of adenovirus early promoters. This E1A antibody recognizes E1A from cells infected with Adenovirus 2 or Adenovirus 5, and recognizes three major bands from 30 - 50 kDa in molecular weight.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use E1A antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with E1A antibody (mAb).

for E1A antibody (mAb) (Clone M73)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for E1A antibody (mAb) (Clone M73)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

E1A antibody (mAb)
 

Data Thumbnails

E1A antibody (mAb) tested by Western blot.

Western blot of E1A mAb.
(Click image to enlarge and see details.)