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Antibodies

Androgen Receptor antibody (pAb)

Aliases: AR, KD, AIS, TFM, DHTR, SBMA, HYSP1, NR3C4, SMAX1, HUMARA
Catalog No: 39781 Format: 100 µl $420 Buy Now
Catalog No: 39782 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:110 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for Androgen Receptor antibody (pAb)Application Notes


Validated Applications:
ChIP: 5 µg per ChIP
ChIP-Seq: 5 µg each
WB: 1 - 2 µg/ml dilution

for Androgen Receptor antibody (pAb)Immunogen

This Androgen Receptor antibody was raised against a peptide in the N-terminal region of human Androgen receptor.

for Androgen Receptor antibody (pAb)Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Androgen Receptor antibody (pAb)References

 
Androgen Receptor antibody (pAb) tested by ChIP-Seq.

Androgen Receptor antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human prostate cancer cell line (3 million cells). ChIP DNA was sequenced on the Illumina GAII and 22 million sequence tags were mapped to identify Androgen Receptor binding sites. The image shows hundreds of strong binding sites dispersed across the right arm of human chromosome 16.

Androgen Receptor antibody (pAb) tested by ChIP.

Androgen Receptor antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of VCAP60 cell chromatin and 10 µg of Androgen receptor antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Androgen Receptor antibody (pAb) tested by Western blot.

Androgen Receptor antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of LnCaP (Lane 1) and DU145 cells (Lane 2) probed with Androgen Receptor antibody (pAb) (1 µg/ml).

for Androgen Receptor antibody (pAb)Background

Androgen Receptor (AR, NR3C4) is a nuclear receptor that facilitates signaling of testosterone and other androgenic hormones in the cytoplasm and translocates into the nucleus upon hormone binding. AR serves as a DNA binding activator of transcription of hormone responsive genes. There are two forms of the Androgen Receptor, the AR-A form, lacking the N-terminal 187 amino acids and the full length AR-B form. Androgen Receptor is involved in the development of both primary and secondary male sexual characteristics and abnormal expression of AR is linked to prostate cancer.

for Androgen Receptor antibody (pAb)Positive Control

Active Motif's LNCaP nuclear extract (Clone FGC) (Catalog No. 36051) can be used as a positive control for Androgen Receptor antibody.

for Androgen Receptor antibody (pAb)qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Negative Control Primer Set 1 (Cat. No. 71011) has been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Androgen Receptor antibody (pAb).

for Androgen Receptor antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Androgen Receptor antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Androgen Receptor antibody (pAb)
 

Data Thumbnails

Androgen Receptor antibody (pAb) tested by ChIP-Seq.

ChIP-Seq of Androgen Receptor pAb.
(Click image to enlarge and see details.)

Androgen Receptor antibody (pAb) tested by ChIP.

ChIP of Androgen Receptor pAb.
(Click image to enlarge and see details.)

Androgen Receptor antibody (pAb) tested by Western blot.

Western blot of Androgen Receptor pAb.
(Click image to enlarge and see details.)