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Antibodies

MITF antibody (mAb)

Clone: C5.D5
Aliases: MI, WS2, CMM8, WS2A, bHLHe32
Catalog No: 39789 Format: 100 µg $420 Buy Now
Catalog No: 39790 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:52-56 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Electrophoretic Mobility Shift Assay Validated Immunocytochemistry Validated

for MITF antibody (mAb)Application Notes


Validated Applications:
ChIP: 2.5 - 10 µg per ChIP
ChIP-Seq: 4 µg each

Published Applications:
IP
WB
ICC/IF
EMSA
See references for more information. Individual optimization may be required.

for MITF antibody (mAb)Immunogen

This MITF antibody was raised against an N-terminal fragment of human MITF.

for MITF antibody (mAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for MITF antibody (mAb)References

 
MITF antibody (mAb) tested by ChIP.

MITF antibody (mAb) tested by ChIP-Seq.
(ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) and chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9.

MITF antibody (mAb) tested by ChIP-Seq.

MITF antibody (mAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human lung melanoma cell line MALME-3M and 4 µg of MITF antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

for MITF antibody (mAb)Background

MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome.

for MITF antibody (mAb)qPCR Primers

Active Motif's Human Negative Control Primer Set 2 (Cat. No. 71002) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Pax-2 (Cat. No. 71020) and Mouse Negative Control Primer Set 3 (Cat. No. 71013) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with MITF antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use MITF antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with MITF antibody (mAb).

for MITF antibody (mAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for MITF antibody (mAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

MITF antibody (mAb)
 

Data Thumbnails

MITF antibody (mAb) tested by ChIP.

ChIP of MITF mAb.
(Click image to enlarge and see details.)

MITF antibody (mAb) tested by ChIP-Seq.

ChIP-Seq of MITF mAb.
(Click image to enlarge and see details.)