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Antibodies

Ki-67 antibody (pAb)

Aliases: MKI67, KIA, MIB-1
Catalog No: 39799 Format: 100 µl $410 Buy Now
Catalog No: 39800 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:360 kDa
Reactivity:Human

Applications

Western Blot Validated

for Ki-67 antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 1:1,000 dilution

for Ki-67 antibody (pAb)Immunogen

This Ki-67 antibody was raised against a peptide within the N-terminal region of human Ki-67.

for Ki-67 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Ki-67 antibody (pAb)References

 
Ki-67 antibody (pAb) tested by Western blot.

Ki-67 antibody tested by Western blot.
Detection of Ki-67 by Western blot. Raji nuclear extract (30 μg) probed with Ki-67 antibody (1:500 dilution). Lane 2: pre-incubation of antibody with immunizing peptide.

for Ki-67 antibody (pAb)Background

Ki-67 encodes a protein that is highly expressed in dividing cells but not in quiescent cells. Ki-67 is a useful marker for rapidly dividing cells and has been used as a marker for cancer cells, Including breast, bladder, brain, colon, and prostate cancer.

for Ki-67 antibody (pAb)Positive Control

Active Motif's Raji nuclear extract (Catalog No. 36023) can be used as a positive control for Ki-67 antibody.

for Ki-67 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Ki-67 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Ki-67 antibody (pAb)
 

Data Thumbnails

Ki-67 antibody (pAb) tested by Western blot.

Western blot of Ki-67 pAb.
(Click image to enlarge and see details.)