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Antibodies

SMARCA2 / BRM antibody (mAb)

Clone: 1H7A10
Aliases: Snf2l2, brahma, SNF2alpha
Catalog No: 39805 Format: 100 µg $410 Buy Now
Catalog No: 39806 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG2a
Purification:Protein G Chromatography
Host:Rat
Molecular Weight:190 kDa
Reactivity:Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

for SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)Application Notes


Validated Applications:
ChIP: 10 µg per ChIP
ICC/IF: 0.5 µg/ml dilution

Published Applications:
ICC/IF
WB
See references for more information. Individual optimization may be required.

for SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)Immunogen

This SMARCA2 / BRM antibody was raised against a recombinant protein corresponding to amino acids 48-214 of mouse BRM.

for SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)References

 
SMARCA2 / BRM antibody (mAb) tested by ChIP.

SMARCA2 / BRM antibody (mAb) tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µg of SMARCA2 / BRM antibody or the equivalent amount of rat IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the CD44 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

SMARCA2 / BRM antibody (mAb) tested by immunofluorescence.

SMARCA2 / BRM antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with SMARCA2 / BRM antibody at a 0.5 µg/ml dilution.

for SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)Background

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of ATP-dependant chromatin remodeling proteins. SMARCA2 is the catalytic subunit of a multi-subunit protein complex that utilizes the energy from ATP hydrolysis to alter chromatin structure and activate transcription.

for SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SMARCA2 / BRM antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SMARCA2 / BRM antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SMARCA2 / BRM antibody (mAb).

for SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for SMARCA2 / BRM antibody (mAb) (Clone 1H7A10)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

SMARCA2 / BRM antibody (mAb)
 

Data Thumbnails

SMARCA2 / BRM antibody (mAb) tested by ChIP.

ChIP of SMARCA2 / BRM mAb.
(Click image to enlarge and see details.)

SMARCA2 / BRM antibody (mAb) tested by immunofluorescence.

Immunofluorescence stain of SMARCA2 / BRM mAb.
(Click image to enlarge and see details.)