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Antibodies

Sp1 non-phospho antibody (pAb)

Aliases: TSFP1
Catalog No: 39809 Format: 100 µl $410 Buy Now
Catalog No: 39810 Format: 10 µl $95 Buy Now

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Antibody Type:Polyclonal
Isotype:IgG
Purification:Affinity Purified
Host:Rabbit
Molecular Weight:96
Reactivity:Human

Applications

Western Blot Validated

for Sp1 non-phospho antibody (pAb)Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

for Sp1 non-phospho antibody (pAb)Immunogen

This Sp1 antibody was raised against a peptide containing serine 101 of human Sp1.

for Sp1 non-phospho antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for Sp1 non-phospho antibody (pAb)References

 
Sp1 non-phospho antibody (pAb) tested by Western blot.

Sp1 non-phospho antibody tested by Western blot.
Detection of unphosphorylated Sp1 by Western blot. Whole-cell extract of U2OS cells (25 µg) probed with Sp1 non-phospho antibody at a dilution of 1:500. Lane 1: No treatment. Lane 2: Cells treated with H2O2.

for Sp1 non-phospho antibody (pAb)Background

Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The Sp1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain.

Sp1 contains two SQ/TQ domains, which are found in proteins phosphorylated by the DNA-damage response transducer/effector kinase ATM. Phosphorylation of serine 101 (dependent upon ATM and ATR) is stimulated by double strand DNA breakage (DSBs) as well as by blocks to DNA replication induced by treatment with hydroxyurea or UV irradiation. Sp1 phospho Ser101 co-localizes with ATM phospho Ser1981 at DSBs, suggesting that this phosphorylated version of Sp1 plays a role in double strand break repair.

for Sp1 non-phospho antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Sp1 non-phospho antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

Sp1 non-phospho antibody (pAb)
 

Data Thumbnails

Sp1 non-phospho antibody (pAb) tested by Western blot.

Western blot of Sp1 non-phospho pAb.
(Click image to enlarge and see details.)