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Oct-4 antibody (pAb)
| Catalog No: 39811 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39812 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:1,000 - 1:1,500 | — |
for Oct-4 antibody (pAb)Background
Oct-4 (Octamer binding protein-4, POU5F1) is a member of the POU family of transcriptional activators. Oct-4 is critical for early embryogenesis and required for embryonic stem cell pluripotency. Oct-4 is expressed at high levels in undifferentiated cells and ectopic expression of Oct-4 (and several other transcription factors) can induce pluripotency in differentiated cells.
for Oct-4 antibody (pAb)Immunogen
This Oct-4 antibody was raised against a peptide derived from human Oct-4.
for Oct-4 antibody (pAb)Application Notes
This Oct-4 antibody has been validated for use in Western blotting (1:1,000 - 1:1,500 dilution). This antibody also detects a protein at 150 kDa and 30 kDa in some lysates.
for Oct-4 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Oct-4 antibody tested by Western blot.
Detection of Oct-4 by Western blot. P19 cell nuclear extract (25 µg) probed with Oct-4 antibody (pab) (1:1,000 dilution).
for Oct-4 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Oct-4 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
