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Ago4 antibody (pAb)
| Catalog No: 39813 | Format: 100 µl | $340 | Buy Now |
| Catalog No: 39814 | Format: 10 µl | $70 | Buy Now |
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Applications Table
| Western Blot | Chromatin IP |
|---|---|
| 1:500 - 1:2,000 | — |
for Ago4 antibody (pAb)Background
Argonaute 4 (Ago4, aka EIF2C4) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago4 lacks RNase activity and does not appear to cleave target mRNA molecules. It binds to microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Ago4 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.
for Ago4 antibody (pAb)Immunogen
This Ago4 antibody was raised against a peptide within the N-terminal region of human Ago4.
for Ago4 antibody (pAb)Application Notes
This Ago4 (Argonaute 4) antibody has been validated for use in Western blotting (1:500 - 1:2,000 dilution).
for Ago4 antibody (pAb)Buffer
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.
Ago4 antibody (pAb) tested by Western blot.
MCF7 whole cell extract (20 µg) probed with Ago4 antibody at a dilution of 1:1,000.
for Ago4 antibody (pAb)Storage
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
for Ago4 antibody (pAb)Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FACS = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting
