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Antibodies

JMJD2A antibody (mAb)

Clone: N154/32
Aliases: KDM4A, JMJD2, JHDM3A, TDRD14A
Catalog No: 39815 Format: 100 µg $410 Buy Now
Catalog No: 39816 Format: 10 µg $95 Buy Now

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Antibody Type:Monoclonal
Isotype:IgG1
Purification:Protein G Chromatography
Host:Mouse
Molecular Weight:130 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for JMJD2A antibody (mAb) (Clone N154/32)Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

for JMJD2A antibody (mAb) (Clone N154/32)Immunogen

This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.

for JMJD2A antibody (mAb) (Clone N154/32)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Also available as an AbFlex™ engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91143.

for JMJD2A antibody (mAb) (Clone N154/32)References

 
JMJD2A antibody (mAb) tested by Western blot.

JMJD2A antibody (mAb) tested by Western blot.
HeLa cell nuclear extract (40 µg) probed with JMJD2A antibody at a dilution of 2 µg/ml.

for JMJD2A antibody (mAb) (Clone N154/32)Background

JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

for JMJD2A antibody (mAb) (Clone N154/32)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JMJD2A antibody.

for JMJD2A antibody (mAb) (Clone N154/32)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for JMJD2A antibody (mAb) (Clone N154/32)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

JMJD2A antibody (mAb)
 

Data Thumbnails

JMJD2A antibody (mAb) tested by Western blot.

Western blot of JMJD2A mAb.
(Click image to enlarge and see details.)