JMJD2A antibody (mAb)

for JMJD2A antibody (mAb) (Clone N154/32)Background

JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.

for JMJD2A antibody (mAb) (Clone N154/32)Immunogen

This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.

for JMJD2A antibody (mAb) (Clone N154/32)Application Notes

This JMJD2A antibody has been validated for use in Western blotting (1 - 2 µg/ml dilution) and immunocytochemistry.

Addition of 0.05% Tween-20 in blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.

for JMJD2A antibody (mAb) (Clone N154/32)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 32 mM glycine, 0.07 mM EDTA, 30% glycerol, and 0.035% sodium azide.

for JMJD2A antibody (mAb) (Clone N154/32)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JMJD2A antibody.

for JMJD2A antibody (mAb) (Clone N154/32)Storage

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for JMJD2A antibody (mAb) (Clone N154/32)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.